Choline-mediated aggregation and dissociation of autolysin of Streptococcus pneumoniae.
نویسندگان
چکیده
منابع مشابه
Cloning of Minor Autolysin of Streptococcus Pneumoniae
Abstract Background and Objective: Increased antibiotic resistant strains and inadequacy of current vaccines against pneumococcal infections necessitate the study of novel protein antigens. It seems that minor autolysin of Streptococcus pneumoniae may have antigenicity. Thus, we aimed at cloning its gene for the first time. Material and Methods: After DNA extraction of Streptococcus pneumoniae ...
متن کاملSpecificity of the autolysin of Streptococcus (Diplococcus) pneumoniae.
A Streptococcus (Diplococcus) pneumoniae autolysin, partially purified from cellular autolysates, was optimally active at pH 7.0 and was stimulated by monovalent cations. Addition of autolysin to walls resulted in the appearance of only N-terminal l-alanine, whereas no glycosidase activity was observed. Walls which had been solubilized by autolysin were separated by gel filtration into a low-mo...
متن کاملautolytic activity and plasma binding study of aap, a novel minor autolysin of streptococcus pneumoniae
pneumococcal autolysins are enzymes involved in cell wall turnover and cellular division physiologically. they have been found to be involved in the pneumococcus pathogenesis. the aim of this study was to identify the autolytic activity of spr1754 as a novel protein of streptococcus pneumoniae . moreover, the binding of the recombinant protein to plasma proteins was also determined. the spr1754...
متن کاملAutolytic Activity and Plasma Binding Study of Aap, a Novel Minor Autolysin of Streptococcus pneumoniae.
Pneumococcal autolysins are enzymes involved in cell wall turnover and cellular division physiologically. They have been found to be involved in the pneumococcus pathogenesis. The aim of this study was to identify the autolytic activity of Spr1754 as a novel protein of Streptococcus pneumoniae. Moreover, the binding of the recombinant protein to plasma proteins was also determined. The spr1754 ...
متن کاملCombinational detection of autolysin and penicillin-binding protein 2B genes of Streptococcus pneumoniae by PCR.
PCR was used to identify penicillin resistance in 1,062 clinical isolates of Streptococcus pneumoniae. Three sets of primers were designed to amplify (i) a 240-bp fragment of the penicillin-binding protein (PBP) 2B gene (pbp2b) of penicillin-susceptible S. pneumoniae (PSSP), (ii) a 215-bp fragment of the class A mutations of the pbp2b gene present in penicillin-resistant S. pneumoniae, and (iii...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1990
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.54.1115